Richard Lundmark - Associate Professor

Membrane sculpting machineries

We study proteins and mechanisms that are required to re-sculpture cellular membranes into highly curved vesicular structures, a necessity for trafficking, compartmentalization and balancing of the membrane integrity in cells. Membrane sculpting is fundamental for many cellular processes and malfunction is implicated in a broad range of human diseases. Our group is focused on understanding the underlying mechanisms for the importance of membrane sculpting during infection, cancer, mental retardation, obesity, and muscle and lipid dystrophies.

Major project areas:

Cell surface dynamics, and the role of endocytic machineries
Biogenesis of replication vesicles by Tick-borne encephalitis virus

We use a wide array of techniques ranging from protein and lipid biochemistry, and structural analysis to advanced high speed imaging of molecules in living cells and correlative imaging approaches for ultra-structural resolution. For imaging, we have established the Biochemical Imaging Centre Umeå (BICU), which is an open access facility.

Group:
Richard Lundmark, Associate Professor
Elin Larsson, Labmanager
Madlen Hubert, Post Doc
Wai Lok Yau, Post doc
 

Former members of the group:
Mikkel Holst, Post Doc
Maite Vidal-Quadras, Post Doc
Van Nguyen-Dinh, post doc
Jagan Mohan, PhD student
Lars Nygård Skalman, PhD student
Jeanette Blomberg, PhD
Monika Francis, PhD
Dana Kahra, PhD
Björn Morén, PhD
Sara Henriksson, PhD
Lionel Tcatchoff, PhD

Publications

Holst MR, Vidal-Quadras M, Larsson E, Song J, Hubert M, Blomberg J, Lundborg M, Landström M and Lundmark R; Clathrin-independent endocytosis suppresses cancer cell blebbing and invasion. Cell Reports. Aug 22, 1893-1905, (2017)

Kindstedt E, Koskinen Holm C, Sulniute R, Martinez-Carrasco I, Lundmark R, and Lundberg P; CCL11, a novel mediator of inflammatory bone resorption, Scientific Reports. (2017)

Hoernke M, Mohan J, Larsson E, Blomberg J, Kahra D, Westenhoff S, Schwieger C, Lundmark R; EHD2 restrains dynamics of caveolae by an ATP‑dependent, membrane-bound, open conformation; Proc Natl Acad Sci U S A. May E4360-E4369 (2017).

Melo AA, Hegde BG, Shah C, Larsson E, Isas JM, Kunz S, Lundmark R, Langen R, Daumke O; Structural insights into the activation mechanism of dynamin-like Eps15-homology domain proteins; Proc Natl Acad Sci U S A. May 30, 5629-5634 (2017).

Vidal-Quadras M, Holst M, R, Francis M, K, Larssson E, Hachimi M, Yau W, L, Peränen J, Martín-Belmonte F and Lundmark R; Endocytic turnover of Rab8 controls cell polarization; Journal of Cell Science. March 1147-1157. (2017).

Vdovikova S, Luhr M, Szalai P, Nygård Skalman L, Francis MK, Lundmark R, Engedal N, Johansson J, Nyunt Wai S; A novel role of Listeria monocytogenes membrane vesicles in inhibition of autophagy and cell death. Frontiers in Cellular and Infection Microbiology. (2017)

Bagnato P, Castagnino A, Cortese K, Bono M, Grasso S, Bellese G, Daniele T, Lundmark R, Defilippi P, Castagnola P and Tacchetti C; Cooperative but distinct early co-signaling events originate from ERBB2 and ERBB1 receptors upon trastuzumab treatment in breast cancer cells; Oncotarget. In press

Storm RJ, Persson BD, Nygård Skalman L, Frängsmyr L, Lindström M, Rankin G, Lundmark R, Pedrosa Domellöf F, Arnberg N; Human adenovirus type 37 uses αVβ1 and α3β1 integrins for infection of human corneal cells; Journal of Virology. Feb 14;91(5) (2017).

Francis MK, Holst MR, Vidal-Quadras M, Henriksson S, Sandblad L and Lundmark R; Endocytic membrane turnover at the leading edge is driven by a transient interaction between Cdc42 and GRAF1.  Journal of Cell Science. 15;128, 4183-95 (2015)

Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Monika Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN; Outer membrane vesicle-mediated export of processed PrtV protease from Vibrio cholera. PLOS one. 29;10(7) (2015)

Mohan J, Morén B, Larsson E, Holst MR, Lundmark R; Cavin3 interacts with cavin1 and caveolin1 to increase surface dynamics of caveolae. Journal of Cell Science. 128, 979-91 (2015)

Olofsson A1, Nygård Skalman L1, Ikenna O, Lundmark R, and Arnqvist A; Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways. mBio. 5 (2014) 1 These authors contributed equally to this work

Shah C, Hegde B, Morén B, Behrmann E, Mielke T, Moenke G1, Spahn C, Richard Lundmark R, Daumke O, Ralf Langen R.; Structural insights into membrane interaction and caveolar targeting of dynamin-like EHD2. Structure, 22 409-20 (2014)

Cortese K, Howes M.T, Lundmark R, Tagliatti E, Bagnato P, Petrelli A, Bono M, McMahon H.T, Parton R.G, Tacchetti C.; The HSP90 inhibitor geldanamycin perturbs endosomal structure and drives recycling ErbB2 and Transferrin to modified MVBs/lysosomal compartments. Molecular Biology of the Cell, 24, 129-44 (2013).

Morén B, Shah C, Howes MT, Schieber NL, McMahon HT, Parton RG, Daumke O, Lundmark R; EHD2 regulates caveola dynamics via ATP-driven targeting and oligomerization. Molecular Biology of the Cell. 23, 1316-29 (2012).

Doherty GJ, Åhlund MK, Howes M, Morén B, Parton RG, McMahon HT, Lundmark R; The endocytic protein GRAF1 is directed to cell matrix adhesion sites and regulates cell spreading. Molecular Biology of the Cell. 22, 4380-9 (2011).

Howes MT, Kirkham M, Riches J, Cortese K, Walser PJ, Simpson F, Hill MM, Jones A, Lundmark R, Lindsay MR, Hernandez-Deviez DJ, Hadzic G, McCluskey A, Bashir R, Liu L, Pilch P, McMahon H, Robinson PJ, Hancock JF, Mayor S, Parton RG; Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells. Journal of Cell Biology. 190, 675-91 (2010)

Lundmark R and Carlsson S, R; Driving membrane curvature in clathrin-dependent and clathrin-independent endocytosis. Seminars in Cell and Developmental Biology, Jun;21(4):363-70. invited peer-reviewed. (2010)

Eberth A, Lundmark R, Gremer L, Dvorsky R, Koessmeier KT, McMahon HT, Ahmadian MR; A BAR domain-mediated auto inhibitory mechanism for the GRAF-family of RhoGAPs. Biochemical Journal 417, 371-7, (2009)

Lundmark R and Carlsson S,R; SNX9: A prelude to vesicular release. Journal of Cell Science 122, 5-11. (2009) invited peer-reviewed

Doherty G and Lundmark R; GRAF-dependent endocytosis. Biochemical Society Transactions. Oct;37, 1061-5. invited peer-reviewed. (2009)

Lundmark R, Doherty GJ, Vallis Y, Howes M, Cortese K, Parton R, McMahon H.T; The GTPase activating protein GRAF1 regulates the CLIC/GEEC endocytic pathway. Current Biology 18, 1802-8, (2008)

Lundmark R, Doherty GJ, Vallis Y, Peter BJ, McMahon H.T; Arf family GTP loading is activated by, and generates, positive membrane curvature. Biochemical Journal 414, 189-194, (2008)

Håberg K, Lundmark R, & Carlsson S.R; SNX18 is an SNX9 paralog that acts as a membrane tubulator in AP-1-positive endosomal trafficking. Journal of Cell Science 121, 1495-505, (2008)

Pylypenko O, Ignatev A, Lundmark R, Rasmuson E, Carlsson SR, Rak A; A combinatorial approach to crystallization of PX-BAR unit of the human Sorting Nexin 9. Journal of Structural Biology 162, 356-60, (2008)

Daumke O1, Lundmark R1, Vallis Y, Martens S, Butler PJ, McMahon H.T; Architecture and mechanistic insight into an EHD ATPase involved in membrane remodeling. Nature 449, 923-927, (2007). 1 These authors contributed equally to this work

Pylypenko O, Lundmark R, Rasmuson E, Carlsson SR, Rak A; Structure of the PX-BAR- membrane remodeling unit of SNX9. EMBO journal 26, 4788-8000, (2007)

Marchès. O, Batchelor. M, Shaw. R.K, Patel. A, Cummings. N, Nagai. T, Sasakawa. C, Carlsson. S.R, Lundmark. R, Cougoule. C, Caron. E, Knutton. S, Connerton. I, and Frankel G; EspF of enteropathogenic E. coli binds sorting nexin 9. Journal of Bacteriology 188, 3110-3115, (2006).

Lundmark. R Carlsson S.; Expression and properties of sorting nexin 9 in dynamin-mediated endocytosis. Methods in Enzymology 404, 545-556, (2005).

Lundmark. R Carlsson. S.; Regulated membrane recruitment of Dynamin-2 is mediated by sorting nexin 9. Journal of Biological Chemistry  279, 42694-702, (2004).

Lundmark. R Carlsson. S.; Sorting nexin 9 participates in clathrin-mediated endocytosis through interactions with the core components. Journal of Biological Chemistry  278, 46772-81, (2003).

Lundmark. R Carlsson. S.; The b-Appendages of the four adaptor-protein (AP) complexes: structure and binding properties, and identification of sorting nexin 9 as an accessory protein to AP-2 Domains. Biochemical  Journal 362, 597-607, (2002).


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Umeå University
Medical Biochemistry and Biophysics
SE-901 87 Umeå, SWEDEN 

Visiting Address
KBC-building, 6th floor

Mobile:  +46 (0)70 620 24 64

Fax:  +46 90 786 9795

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